The Global View of miRNA: Strategies for Visualizing miRNA Expression
Posted February 07, 2007
MicroRNAs (miRNAs), a class of small RNA molecules that are 21–23 nucleotides in length, are the newest addition to the repertoire of regulatory factors that control gene expression. The distinguishing feature of miRNAs is that they arise from precursor molecules that are 70-100 nucleotides long with a characteristic stem-loop structure. Unlike polypeptide transcriptional factors, miRNAs are not active in the nucleus, but are active in the cytoplasm where they regulate gene expression after transcription.
On October 3, 2006 the RNAi Discussion Group held a meeting focused on ways to identify and determine the function of miRNAs. Topics discussed included computational and direct cloning approaches for cataloging miRNAs, several methods for verifying the expression of candidate miRNAs, and efforts to identify miRNAs involved in specific developmental processes.
Use the tabs above to find a meeting report and multimedia from this event.
This web site hosts a comprehensive and searchable database of published miRNA sequences.
GeneTools is the sole supplier of research quantities of morpholinos. The web site provides information about the applications of morpholinos in miRNA research.
454 Life Sciences
454 Life Sciences provides services and instrumentation for high-throughput deep DNA sequencing.
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Drosophila microRNAs: From Genes to Functions
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MicroRNA Expression and Functional Analysis
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Cell-type and Tissue-specific miRNAs Determined by Small RNA Library Cloning
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Tools and Strategies to Analyze miRNAs
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Eric Lai, PhD
Eric Lai is an assistant member of the Department of Developmental Biology at Memorial Sloan-Kettering Cancer Center and assistant professor in the program in Cell Biology and Genetics at Weill Cornell Medical School. He received his PhD in biology from the University of California, San Diego. He was a postdoctoral fellow at HHMI/Department of Molecular and Cell Biology in the laboratory of Gerald Rubin at the University of California, Berkeley. Lai received the Career Award in the Biomedical Sciences from the Burroughs Wellcome Fund in 2005 and was a special fellow of the Leukemia and Lymphoma Society from 2004 to 2006.
Wigard Kloosterman studied biology and received a master in biotechnology at the University of Groningen, The Netherlands (1998–2003). In August 2003, he began his graduate studies in the lab of Ronald Plasterk at the Hubrecht Laboratory, Utrecht, The Netherlands, where he is studying microRNA expression and function in the zebrafish embryo.
Pablo Landgraf, MD, PhD
Pablo Landgraf is a postdoctoral fellow in the laboratory of Thomas Tuschl at the Rockefeller University. Landgraf received his MD from the Ludwig- Maximilians- University (LMU) of Munich in 2001. In 2003 he received his PhD from the Department of Clinical Biochemistry at Haunersches Children's Hospital, University of Munich. His thesis was titled "The identification of the peroxisomal targeting signal of the Adrenoleukodystrophy Protein partially conserved within the family of peroxisomal ABC-Transporters." Landgraf's current research is miRNA expression profiling in mammals.
Zissimos Mourelatos, MD
Zissimos Mourelatos is assistant professor of pathology and laboratory medicine at the University of Pennsylvania School of Medicine. He studies the basic biology of small regulatory RNAs and how misregulation of RNA pathways contributes to motor neuron degeneration. Mourelatos has received the Michael S. Brown New Investigator Research Award for his work. His lab has developed novel technologies to profile miRNAs and to study their cellular and subcellular distribution in healthy and diseased human tissue. Mourelatos received his MD from the Aristotelian University of Thessaloniki (Greece) in 1991.
Eva Derman is a molecular biologist and science writer based in New York City. For 20 years she was Head of Laboratory and Associate Member of the Department of Developmental and Structural Biology at the Public Health Research Institute, where she conducted research on gene structure and regulation, molecular immunology, molecular endocrinology, and other topics.